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Related Experiment Video

Updated: May 19, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Association between P2X7 Receptor Polymorphisms and Bone Status in Mice.

Susanne Syberg1, Peter Schwarz, Solveig Petersen

  • 1Research Centre of Ageing and Osteoporosis, Departments of Diagnostics and Medicine, Glostrup University Hospital, 2600 Glostrup, Denmark.

Journal of Osteoporosis
|August 25, 2012
PubMed
Summary

Mice with a specific mutation in the purinergic receptor P2X7 (P451L) show altered bone density and resorption. This finding impacts research on P2X7

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Area of Science:

  • Bone Biology
  • Immunology
  • Genetics

Background:

  • Purinergic receptor P2X7 (P2X7R) is expressed in bone cells and influences bone physiology.
  • A naturally occurring mutation, P451L, impairs P2X7R function in macrophages.
  • The bone phenotype associated with the P451L mutation in P2X7R is not well understood.

Purpose of the Study:

  • To investigate the bone phenotype of mouse strains harboring the P451L mutation in the purinergic receptor P2X7 gene.
  • To determine the impact of P2X7R variants on bone density, strength, and resorption markers.
  • To assess the in vitro osteoclast function related to ATP-induced pore formation.

Main Methods:

  • Sequencing of common inbred mouse strains to identify P2X7R genotypes (P451 vs. P451L).

Related Experiment Videos

Last Updated: May 19, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

  • Comparative analysis of bone phenotype using dual energy X-ray absorptiometry (DXA), bone markers (CTX), and three-point bending tests.
  • In vitro assessment of ATP-induced pore formation in osteoclasts derived from different mouse strains.

    • Main Results:

    • Mouse strains with the P451 allele exhibited stronger femurs and higher C-telopeptide collagen (CTX) levels, indicating increased bone resorption.
    • Strains carrying the P451L allele displayed weaker bones and lower CTX levels, suggesting reduced bone resorption.
    • Osteoclasts from strains with the P451L allele showed diminished ATP-induced pore formation in vitro.

    Conclusions:

    • The P451L mutation in purinergic receptor P2X7 is associated with a distinct bone phenotype characterized by weaker bones and lower resorption.
    • Decreased ATP-induced pore formation in osteoclasts may underlie the observed bone phenotype in P451L mutant strains.
    • These findings are crucial for interpreting previous P2X7R studies in mice and for developing P2X7R-targeted therapies for conditions like postmenopausal osteoporosis.