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Updated: May 19, 2026

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
Comparative genomic analysis of Escherichia coli O104:H4 stx2 prophage reveals a potential new method to identify
Pengcheng Du1, Wen Zhang, Haiyin Wang
1State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Abstract:
This study investigated the process of virulence acquisition in a Shiga toxin-producing Escherichia coli O104:H4 strain, TY-2482, by comparing the sequence of stx2 (Shiga toxin 2) prophage with the sequences of 1421 complete bacterial genomes and analyzing the evolutionary relationships of the stx2 prophage-like regions identified. The results showed a strong positive correlation between the evolutionary distance and the distance to switch sites of GC skew, suggesting that the loci near these sites are "hotspots" for insertions. These findings represent a potential new method to identify virulence factors in bacterial genomes.
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