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Decreased Body Temperature

Decreased Body Temperature

A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by sustained extreme cold exposure, and severe...

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Related Experiment Video

Updated: May 19, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Hypothalamic transcript profiling in hypothermia using SuperSAGE.

Masataka Takamiya¹, Nori Nakayashiki, Koji Dewa

¹Department of Legal Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate 028-3694, Japan. mtakamiy@iwate-med.ac.jp

Journal of Forensic and Legal Medicine

|August 28, 2012

Summary

This summary is machine-generated.

Investigating hypothalamic gene expression in hypothermia revealed significant transcriptomic differences. These molecular insights using SuperSAGE offer potential for developing new diagnostic biomarkers for hypothermia.

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Area of Science:

Molecular Biology
Genomics
Forensic Pathology

Background:

Understanding hypothermia's molecular basis is crucial for diagnosis.
Hypothalamic gene expression changes during hypothermia are not fully understood.

Purpose of the Study:

To investigate the hypothalamic transcriptome in hypothermia.
To identify differentially expressed genes as potential diagnostic biomarkers.

Main Methods:

Utilized SuperSAGE (Serial Analysis of Gene Expression) for high-throughput transcriptomic analysis.
Collected 62,208 and 54,084 tags from normal and hypothermic hypothalami, respectively.
Validated findings for selected genes using quantitative PCR.

Main Results:

Identified 367 statistically significant differentially expressed transcripts.
157 transcripts were upregulated in normal hypothalami, and 210 in hypothermic hypothalami.
SuperSAGE and quantitative PCR results showed consistency for selected genes.

Conclusions:

Hypothalamic mRNA expression patterns are valuable for hypothermia diagnosis.
SuperSAGE is a promising method for gene discovery and transcriptomic analysis, particularly in forensic pathology.