Developmental validation of the PowerPlex® 18D System, a rapid STR multiplex for analysis of reference samples

Area of Science:

• Forensic Science
• Genetics
• Molecular Biology

Background:

• Short tandem repeat (STR) markers are fundamental for global human identification.
• New STR multiplex systems require thorough validation for reliability and robustness.

Purpose of the Study:

• To validate the PowerPlex® 18D System for direct amplification of buccal and blood samples.
• To assess the system's performance for human identification testing according to SWGDAM guidelines.

Main Methods:

• Testing included sensitivity, concordance, inhibitor tolerance, and performance under varying thermal cycling and reaction conditions.
• The PowerPlex® 18D System amplifies 13 CODIS loci, amelogenin, Penta E, Penta D, D2S1338, and D19S433.
• Direct amplification from FTA® storage cards and other sample materials was accommodated.

Main Results:

• The PowerPlex® 18D System demonstrated high sensitivity and concordance across tested parameters.
• The assay showed tolerance to inhibitors and consistent performance with variations in cycling and reaction conditions.
• Validation confirmed the system's suitability for forensic and human identification applications.

Conclusions:

• The PowerPlex® 18D System is a reliable and robust tool for human identification.
• The validated performance supports its confident use in forensic DNA testing.
• This system enhances capabilities for genotyping essential forensic STR loci.