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Related Concept Videos

Influenza01:27

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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Related Experiment Video

Updated: May 19, 2026

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
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Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

Engineering influenza viral vectors.

Junwei Li1, Maria T Arévalo, Mingtao Zeng

  • 1Center of Excellence for Infectious Diseases, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX, USA.

Bioengineered
|August 28, 2012
PubMed
Summary
This summary is machine-generated.

Reverse genetics enables the creation of modified influenza viruses. This review details the evolution of influenza virus reverse genetics systems, from early methods to current single-plasmid systems for diverse applications.

Keywords:
gene deliverygene therapyinfluenza virusmicroRNA deliverypseudo-typed virusreverse geneticsvaccineviral vector

More Related Videos

Generation of Recombinant Influenza Virus from Plasmid DNA
11:31

Generation of Recombinant Influenza Virus from Plasmid DNA

Published on: August 3, 2010

Related Experiment Videos

Last Updated: May 19, 2026

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
10:39

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

Generation of Recombinant Influenza Virus from Plasmid DNA
11:31

Generation of Recombinant Influenza Virus from Plasmid DNA

Published on: August 3, 2010

Area of Science:

  • Virology
  • Molecular Biology
  • Genetic Engineering

Background:

  • Influenza virus, a segmented RNA pathogen, has been a focus of laboratory research since the 1980s.
  • Early advancements enabled the construction of recombinant influenza viruses using cDNA plasmid systems.
  • Reverse genetics techniques have revolutionized the study and manipulation of influenza viruses.

Purpose of the Study:

  • To review the historical development of influenza virus reverse genetics.
  • To highlight the progression from helper virus-dependent to helper virus-independent systems.
  • To showcase applications of modified influenza viruses in vaccine and gene therapy development.

Main Methods:

  • Discussion of the evolution of reverse genetics systems for influenza virus.
  • Comparison of helper virus-dependent and helper virus-independent plasmid systems (17-, 3-, and 1-plasmid systems).
  • Review of successes in modifying viral gene segments.

Main Results:

  • Demonstration of a clear evolutionary path in reverse genetics systems for influenza virus.
  • Successful development of increasingly simplified plasmid systems (from 17 to 1 plasmid).
  • Highlighting successful modifications of influenza virus gene segments.

Conclusions:

  • Reverse genetics has significantly advanced influenza virus research and manipulation.
  • The development of simplified plasmid systems has facilitated broader applications.
  • Modified influenza viruses hold promise for vaccine and gene therapy innovations.