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Updated: May 19, 2026

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
Published on: March 6, 2019
Synthesis of oligonucleotides carrying thiol groups using a simple reagent derived from threoninol
Sonia Pérez-Rentero1, Santiago Grijalvo, Rubén Ferreira
1Institute for Research in Biomedicine-IRB Barcelona, Baldiri Reixac 10, E-08028 Barcelona, Spain.
Abstract:
Oligonucleotides carrying thiol groups are useful intermediates for a remarkable number of applications involving nucleic acids. In this study, DNA oligonucleotides carrying tert-butylsulfanyl protected thiol groups have been prepared. A building block derived from threoninol has been developed to introduce a thiol group at any predetemined position of an oligonucleotide. The resulting thiolated oligonucleotides have been used for the preparation of oligonucleotide conjugates and for the functionalization of gold nanoparticles using the reactivity of the thiol groups.
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