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Published on: February 20, 2015
Chicken IL-17F: identification and comparative expression analysis in Eimeria-infected chickens
Woo H Kim1, Jipseol Jeong, Ae R Park
1College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Developmental and Comparative Immunology
|August 28, 2012
Summary
Chicken Interleukin-17F (chIL-17F) is a unique proinflammatory cytokine identified in chicken splenic lymphocytes. Its expression is upregulated in the gut during parasitic infections, suggesting a distinct role in avian immunity.
Area of Science:
- Immunology
- Cytokine Biology
- Avian Biology
Background:
- Interleukin-17F (IL-17F) is a key proinflammatory cytokine involved in maintaining gut homeostasis.
- Understanding IL-17F in avian species is crucial for avian immunology and disease resistance.
Purpose of the Study:
- To identify and characterize chicken IL-17F (chIL-17F).
- To investigate the expression profile of chIL-17F in various tissues and under different immune stimuli.
- To compare chIL-17F with chIL-17A and mammalian IL-17 homologues.
Main Methods:
- Identification of chIL-17F cDNA from ConA-activated chicken splenic lymphocytes.
- Sequence analysis and comparison with known IL-17 family members.
- Quantitative real-time RT-PCR to examine chIL-17F and chIL-17 expression.
- Assessment of recombinant chIL-17F biological activity in primary chicken embryonic fibroblasts.
Main Results:
- A full-length chIL-17F cDNA was identified, sharing 53% amino acid identity with chIL-17A.
- chIL-17F transcript expression was high in lymphoblast cell lines and moderate in intestinal tissues and liver.
- chIL-17F expression was upregulated in mitogen-stimulated lymphocytes and intestinal tissues infected with Eimeria species, unlike chIL-17A.
- Recombinant chIL-17F exhibited biological activity similar to chIL-17.
Conclusions:
- chIL-17F is a distinct member of the IL-17 cytokine family in chickens.
- chIL-17F plays a significant role in the chicken immune response, particularly in the gut during parasitic infections.
- The findings provide insights into the evolution and function of IL-17 cytokines in avian species.
