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Updated: May 19, 2026
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Development of a novel primer combination to detect pathogenic Leptospira by loop-mediated isothermal amplification
D Suwancharoen1, C Kulchim, C Chirathaworn
1National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand. duangjais@dld.go.th
Abstract:
Loop-mediated isothermal amplification (LAMP) has been used for the detection of leptospiral 16S rDNA. Here we report the development of the LAMP method for leptospiral 16S rDNA detection which can be interpreted easily within 90 min by visualizing the fluorescence of calcein. The lower limit of detection (LLOD) is 10-100 copies.
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