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Updated: May 19, 2026

Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
Published on: July 3, 2018
Toluidine blue: A review of its chemistry and clinical utility
Gokul Sridharan1, Akhil A Shankar
1Department of Oral Pathology and Microbiology, YMT Dental College and Hospital, Kharghar, Navi Mumbai, Maharashtra, India.
Abstract:
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property. Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage. This article provides an overview on chemistry, technique, and the various applications of toluidine blue.
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