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Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental
G Obando Pereda1, A C Cornejo Fudinaga, H Salas Beltrán
1Department of Anatomy, Cell Biology and Physiology and Biophysics, University of Campinas, Sao Paulo, Brazil. obando@unicamp.br
Background:
Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-α, IL-1β and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures.
Methods:
Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA.
Results:
The expression of TNF-α for both commercial and experimental MTA was higher, while the expression of IL-1β and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control.
Conclusions:
This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity.
