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Updated: May 19, 2026
07:16
DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
Published on: February 9, 2024
Gold nanoparticle-enabled real-time ligation chain reaction for ultrasensitive detection of DNA
Wei Shen1, Huimin Deng, Zhiqiang Gao
1Department of Chemistry, National University of Singapore, Singapore 117543.
Journal of the American Chemical Society
|August 29, 2012
Abstract:
A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).
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