Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EZH2 Serine 21 Phosphorylation Restrains Compact-State PRC2 Activation and H3K27me3 Propagation.

bioRxiv : the preprint server for biology·2026
Same author

Cleavage of histone H2A during embryonic stem cell differentiation destabilizes nucleosomes to counteract gene activation.

The Journal of biological chemistry·2026
Same author

Deep learning the dynamic regulatory sequence code of cardiac organoid differentiation.

bioRxiv : the preprint server for biology·2025
Same author

PBRM1-Dependent PBAF Targeting is Required for EMT and Metastasis in Breast Cancer.

bioRxiv : the preprint server for biology·2025
Same author

Surface Iodide Defects Control the Kinetics of the CsPbI<sub>3</sub> Perovskite Phase Transformation.

ACS energy letters·2024
Same author

Simulating Metal-Imidazole Complexes.

Journal of chemical theory and computation·2024

Related Experiment Video

Updated: May 19, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Wide dynamic range sensing with single quantum dot biosensors.

Stacey R Opperwall1, Anand Divakaran, Elizabeth G Porter

  • 1Department of Chemistry & Biochemisty, Calvin College, 1726 Knollcrest Circle SE, Grand Rapids, Michigan 49546, United States.

ACS Nano
|August 29, 2012
PubMed
Summary
This summary is machine-generated.

Charge-transfer biosensors using semiconductor nanoparticles offer ultrasensitive detection of analytes like maltose. Single-particle analysis reveals a wide dynamic range and constant emission for real-time monitoring in living cells.

More Related Videos

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Related Experiment Videos

Last Updated: May 19, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Biosensors are crucial for detecting analytes, with semiconductor nanoparticles (quantum dots) offering unique signaling properties.
  • Existing energy-transfer biosensors exhibit analyte-dependent on/off switching of nanoparticle emission.
  • Charge-transfer biosensors present an alternative signaling mechanism with constant emission, modulated by analyte presence.

Purpose of the Study:

  • To investigate single-particle behavior of charge-transfer-based biosensors utilizing semiconductor nanoparticles.
  • To characterize the analyte-dependent signaling and dynamic range of these novel biosensors.
  • To explore the potential for ultrasensitive, real-time detection of small molecules in biological systems.

Main Methods:

  • Single-particle analysis of immobilized charge-transfer biosensors.
  • Utilizing semiconductor nanoparticles (quantum dots) functionalized with maltose binding protein.
  • Monitoring changes in nanoparticle emission intensity in response to varying maltose concentrations.

Main Results:

  • Charge-transfer biosensors demonstrated constant emission with analyte-dependent intensity increases.
  • A single biosensor construct exhibited a wide dynamic range for maltose detection (100 pM to 10 μM).
  • Single-particle tracking revealed heterogeneous responses but enabled quantifiable, real-time maltose monitoring.

Conclusions:

  • Charge-transfer biosensors provide a robust platform for ultrasensitive analyte detection.
  • Single-particle analysis is key to understanding and optimizing heterogeneous biosensor responses.
  • These biosensors hold promise for real-time intracellular detection of small molecules.