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Related Experiment Videos

Simian rotavirus SA11 replication in cell cultures.

M K Estes, D Y Graham, C P Gerba

    Journal of Virology
    |September 1, 1979
    PubMed
    Summary

    This study details simian rotavirus SA11 replication in cell cultures, identifying optimal cell lines for high-titer viral growth. Findings advance understanding of rotavirus (RV) infection dynamics and assay development.

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    Epidemiology and infection·2015

    Area of Science:

    • Virology
    • Cell Biology
    • Infectious Diseases

    Background:

    • Rotavirus infections pose significant global health challenges.
    • Studying rotavirus (RV) virology is hindered by isolate fastidiousness and lack of rapid quantitative assays.

    Purpose of the Study:

    • To analyze the growth characteristics and replication of simian rotavirus SA11 in various cell cultures.
    • To identify cell lines supporting high-titer RV growth and quantify infectivity.

    Main Methods:

    • Simian rotavirus SA11 replication was assessed across diverse primary cell cultures and continuous cell lines.
    • Viral infectivity was quantitated using plaque assays.
    • Growth curves were generated for SA11 in selected cell cultures.

    Main Results:

    • Rotavirus SA11 replicated in all examined cell cultures, with notable variability in susceptibility.
    • MA104, BSC-1, CV-1, and BGM cells yielded the highest viral titers.
    • Cytopathic effects correlated with the percentage of infected cells.

    Conclusions:

    • Cell culture systems provide a viable method for studying rotavirus SA11 replication.
    • Specific cell lines, particularly MA104, are highly suitable for high-titer simian rotavirus SA11 propagation.
    • This research facilitates the development of improved rotavirus diagnostic and research tools.

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