Related Experiment Video
Updated: May 19, 2026
Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
Published on: August 4, 2021
Assessment of sperm antigen specific T regulatory cells in women with recurrent miscarriage
Chaodong Liu1, Xian-Zhong Wang, Xin-Bo Sun
1Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. chaodongliu7@163.com
Background And Aims:
The prevalence of recurrent miscarriage (RM) is about 1-3% of women; the pathogenesis of RM is not fully understood yet. This study aims to assess the sperm antigen specific regulatory T cells (Treg) in women with RM.
Methods:
A group of women with RM was recruited into this study. The sperm antigen was extracted from the semen samples of each woman's husband. The sperm antigen specific T cell response was assessed by flow cytometry.
Results:
Low frequency of sperm specific Tregs and high frequency of T helper (Th)1 cells were detected in RM women as compared with women without RM. The sperm specific Tregs in RM women expressed less Ubc13. Knockdown of Ubc13 from Tregs converted the Tregs to effector T cells.
Conclusions:
Immune deregulation may play an important role in RM.
More Related Videos
07:46Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis
Published on: October 13, 2023
08:29Measurement of Four Uterine NK Cell Subtypes Using Multiplexed Fluorescent Immunohistochemical Staining in Women with Repeated Implantation Failure
Published on: October 25, 2024