SB202190 affects cell response to hydroxyurea-induced genotoxic stress in root meristems of Vicia faba

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Konrad Krajewski1, Janusz Maszewski

  • 1Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz, ul. Pomorska 141/143, 90-236 Lodz, Poland. winnicki@biol.uni.lodz.pl

Plant Physiology and Biochemistry : PPB
|August 29, 2012
PubMed

Insights

Mitogen-activated protein kinases (MAPKs) and ATM/ATR pathways cooperate in responding to genotoxic stress. This interaction influences cell cycle checkpoints and DNA repair in Vicia faba root meristem cells.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Genotoxic stress can cause DNA breaks and genome instability.
  • ATM/ATR sensor kinases and their downstream proteins regulate cell cycle checkpoints in response to DNA damage.
  • Mitogen-activated protein kinases (MAPKs) are also activated by genotoxic stress and can influence checkpoint protein function.

Purpose of the Study:

  • To investigate the role of the MAP kinase signaling pathway in the response to hydroxyurea-induced genotoxic stress in Vicia faba root meristem cells.
  • To explore the potential cooperation between MAP kinase signaling pathways and ATM/ATR-dependent checkpoints.

Main Methods:

  • Utilized Vicia faba root meristem cells.
  • Applied hydroxyurea (HU) to induce genotoxic stress.
  • Used SB202190, a p38 MAP kinase inhibitor, and caffeine to modulate signaling pathways.
  • Assessed premature chromosome condensation (PCC) and histone H4 Lys5 acetylation.

Main Results:

  • MAP kinase signaling pathway participates in the response to hydroxyurea-induced genotoxic stress.
  • SB202190 (p38 inhibitor) accelerated PCC when cell cycle checkpoints were alleviated by caffeine.
  • Both SB202190 and caffeine reduced HU-mediated histone H4 Lys5 acetylation.

Conclusions:

  • MAP kinase signaling pathways and ATM/ATR-dependent checkpoints likely cooperate in the cellular response to genotoxic stress.
  • This cooperation may involve the regulation of histone modifications and cell cycle progression.

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