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Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
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Published on: April 29, 2011

CCN5 Expression in mammals. III. Early embryonic mouse development.

Ronald B Myers1, Kibibi Rwayitare, Lauren Richey

  • 1Program in Cell, Molecular, and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA, USA.

Journal of Cell Communication and Signaling
|August 29, 2012
PubMed
Summary

Cellular communication network factor 5 (CCN5) is crucial for early mammalian development. CCN5 mRNA is first detected at the early morula stage and widely expressed in early embryos, suggesting a vital developmental role.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Cellular communication network (CCN) proteins are vital for fundamental cellular processes.
  • CCN5 is known to be expressed from E9.5 to adulthood in mice.
  • Previous studies indicated early embryonic lethality in CCN5 knockout and over-expressing mice.

Purpose of the Study:

  • To characterize the expression pattern of CCN5 in preimplantation and early post-implantation murine embryos.
  • To investigate the role of CCN5 in early mammalian development.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect CCN5 mRNA in preimplantation embryos (1-cell to blastocyst stages).
  • Immunohistochemical staining was employed to visualize CCN5 protein distribution in post-implantation embryos (E4.5 to E7.5).

Main Results:

  • CCN5 mRNA was absent in 1-cell, 2-cell, and 4-cell embryos.
  • CCN5 mRNA was first detected at the early morula stage and continued through the blastocyst stage.
  • CCN5 protein was widely distributed across all germ layers and cell types in post-implantation embryos.

Conclusions:

  • CCN5 exhibits a dynamic expression pattern during early embryogenesis, starting from the morula stage.
  • The widespread distribution of CCN5 in early embryonic stages highlights its significant role in mammalian development.
  • Further research is warranted to elucidate the specific functions of CCN5 in developmental processes.