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Updated: May 19, 2026
Neurogenesis Using P19 Embryonal Carcinoma Cells
Published on: April 27, 2019
[Establishment of optimized neuronal differentiation-promoting model derived from P19 embryonic carcinoma cells]
Yu-qin Mei1, Xing-guang Liang, Yi-jia Lou
1Division of Cardio-Cerebral Vascular and Hepatic Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Objective:
To establish an optimized primary drug screen model of neuronal differentiation using P19 embryonal carcinoma cells.
Methods:
The final concentration of retinoid acid (RA), days of suspension culture, manner of adherent culture, suitable cell density and adherent culture medium were tested, respectively. Two stages of neuronal differentiation were examined based on morphological changes and immunocytochemistry analysis of neuronal specific protein β-tubulin III.
Results:
On d 8 of differentiation culture, neuron-like cells were observed with final concentration of 1 μmol/L RA. Neuron-like network was formed on d 16 of neuronal differentiation. β-tubulin III was positively stained on both stages, indicating P19 cells were differentiated into neurons.
Conclusion:
The model using RA to induce P19 embryonic carcinoma cells to differentiate into neuron-like cells has been successfully established, which may provide a rapid, phenotypic cell-based platform for primary screening of neurogenesis-promoting drugs.
