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[Screening and identification of high-yield poly(β-malic acid) bacterial strain]
Ling-fen Xu1, Zhong-hua Chen, Jing Zhao
1School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China.
Objective:
To isolate and identify the high-yield poly-malic acid (PMLA) bacterial strains from the nature.
Methods:
Samples were collected and cultured. The high-yield PMLA bacterial strains were screened through morphological observation, qualitative PMLA tests by HPLC and ITS sequence analysis on the isolated bacterial strains.
Results:
A high-yield PMLA strain II 04 was isolated, the yield of PMLA of the strain reached to 26.23g/L in the rotary shaker at 25 degree for 7d. From morphological observation and ITS sequences analysis, the strain belonged to Aureobasidium pullulans, and named as Aureobasidium pullulans ZUCC-41.
Conclusion:
A high-yield bacterial strain has been isolated from the nature and identified to be Aureobasidium pullulans.
