Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Noncompartmental Analysis: Mean Transit, Absorption and Dissolution Time

Noncompartmental Analysis: Mean Transit, Absorption and Dissolution Time

When drugs are administered extravascularly, a comprehensive evaluation through noncompartmental analysis becomes imperative. This analytical approach considers various parameters that play a crucial role in understanding the pharmacokinetics of these drugs.
One of the key parameters is the mean transit time (MTT), which refers to the total duration required for drug molecules to transit through the body. MTT is determined by calculating the ratio of the area under the moment curve to the area...

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...

Chronopharmacokinetics: Time-Dependent Pharmacokinetics

Chronopharmacokinetics: Time-Dependent Pharmacokinetics

Chronopharmacokinetics studies the temporal change in drug absorption and elimination. These changes can be cyclical or non-cyclical. Cyclical changes occur over a regular interval, while non-cyclical changes occur over a longer, irregular period.
Time-dependent pharmacokinetics refers to non-cyclical changes in drug rate processes over a period of time. It can lead to nonlinear pharmacokinetics, where the relationship between drug concentration and time is not proportional. Non-cyclical...

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

Measurement of Bioavailability: Pharmacokinetic Methods

Measurement of Bioavailability: Pharmacokinetic Methods

Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...

Metabolic States of the Body: The Absorptive State

Metabolic States of the Body: The Absorptive State

During the absorptive state, which lasts approximately four hours after a meal, the body absorbs nutrients from the gastrointestinal tract. The carbohydrates, proteins, and lipids we consume are broken down into monosaccharides, amino acids, and free fatty acids for absorption. While carbohydrates and proteins are absorbed as-is, lipids are absorbed in their broken-down forms and then re-esterified into triglycerides within enterocytes before being packaged into chylomicrons. These absorbed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Poor sleep quality correlates with axial symptoms and mood problems in Parkinson's disease.

Dialogues in clinical neuroscience·2026

Same author

Time-stratified daily walking speed measurement via smartphone and its predictive utility for mild cognitive impairment.

Scientific reports·2026

Same author

Adult Bochdalek hernia incarcerated after laparoscopic nephrectomy: a case report.

Journal of surgical case reports·2026

Same author

Usefulness of a Video of a Gatekeeper Training Program for Suicide Prevention among University Students on Campus: Randomized Controlled Trial.

The Tohoku journal of experimental medicine·2026

Same author

Prescription patterns of hypnotics for children and adolescents in Japan: a descriptive epidemiologic study using a claims database.

Sleep·2026

Same author

Preventive circadian medicine: improving health with sleep checkups.

Npj biological timing and sleep·2026

Search research articles

Related Experiment Video

Updated: May 19, 2026

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

Published on: October 6, 2023

Human blood metabolite timetable indicates internal body time.

Takeya Kasukawa¹, Masahiro Sugimoto, Akiko Hida

¹Functional Genomics Unit, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo 650-0047, Japan.

Proceedings of the National Academy of Sciences of the United States of America

|August 29, 2012

Summary

Accurately estimate internal body time using a novel metabolite timetable derived from human blood. This minimally invasive method aids personalized medicine, optimizing drug and feeding schedules for maximum efficacy and minimal toxicity.

More Related Videos

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Published on: August 8, 2019

Related Experiment Videos

Last Updated: May 19, 2026

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

Published on: October 6, 2023

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Published on: August 8, 2019

Area of Science:

Biomedical Science
Chronobiology
Metabolomics

Background:

Accurate estimation of internal body time (BT) is crucial for chronotherapy and time-restricted feeding.
Previous methods relied on complex protocols or animal models.
A convenient and accurate method for human BT estimation is needed.

Purpose of the Study:

To develop and validate a molecular timetable for estimating internal body time in humans using blood samples.
To assess the accuracy and feasibility of this method under controlled conditions.

Main Methods:

Constructed a 1.5-day reference timetable of oscillating metabolites in human blood samples.
Collected blood samples every 2 hours with a 2-hour sampling frequency.
Controlled for confounding factors including activity, light, temperature, sleep, and food intake.

Main Results:

Developed a reference timetable of oscillating metabolites in human blood.
Accurately determined internal body time within a 3-hour window using just two anti-phase blood samples.
Demonstrated the method's robustness by controlling for major confounding variables.

Conclusions:

The molecular timetable method provides a convenient and accurate way to estimate human internal body time.
This minimally invasive approach using blood samples enables highly optimized and personalized medicine.
The findings support the application of this method in chronotherapy and time-restricted feeding strategies.