Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging in scanning microscopes with slit-shaped detectors.

T Wilson1, S J Hewlett

  • 1University of Oxford, U.K.

Journal of Microscopy
|November 1, 1990
PubMed
Summary

Scanning microscopes with slit detectors produce asymmetrical images at low defocus levels. Image quality degrades with increased defocus, though some extended-focus images remain acceptable.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bed occupancy and nosocomial infections in the intensive care unit: A retrospective observational study in a tertiary hospital.

The Southern African journal of critical care : the official journal of the Critical Care Society·2024
Same author

Minimally invasive cerebral revascularization in moyamoya disease in adult patients.

Neuro-Chirurgie·2022
Same author

Post-operative volumes following endoscopic surgery for non-functioning pituitary macroadenomas are predictive of further intervention, but not endocrine outcomes.

BMC endocrine disorders·2021
Same author

Treatment Preferences for Cardiac Procedures of Patients With Chronic Kidney Disease in Acute Coronary Syndrome: Design and Pilot Testing of a Discrete Choice Experiment.

Canadian journal of kidney health and disease·2021
Same author

Addressing the pitfalls when designing intervention studies to discover and validate biomarkers of habitual dietary intake.

Metabolomics : Official journal of the Metabolomic Society·2019
Same author

Purified Human Pancreatic Islets, CIT Culture Media with Lisofylline or Exenatide.

CellR4-- repair, replacement, regeneration, & reprogramming·2019

Area of Science:

  • Optical microscopy
  • Image processing

Background:

  • Scanning microscopes utilize various detector shapes for imaging.
  • Understanding the impact of detector geometry and defocus on image quality is crucial.

Purpose of the Study:

  • To analyze the 3D imaging characteristics of scanning microscopes with point and slit-shaped detectors.
  • To evaluate image quality in bright-field and fluorescent modes under varying defocus conditions.

Main Methods:

  • Theoretical analysis of imaging systems with point and slit detectors.
  • Simulation of image formation in scanning microscopy.
  • Assessment of image asymmetry and quality metrics at different defocus levels.

Main Results:

  • Slit-shaped detectors yield acceptable but asymmetrical images at low defocus.
  • Increased defocus significantly degrades image quality for slit detectors.
  • Acceptable extended-focus and isometric images are achievable in specific cases despite defocus.

Conclusions:

  • Detector shape critically influences image fidelity in scanning microscopy.
  • Defocus management is essential for optimizing imaging with slit detectors.
  • Careful selection of imaging parameters can mitigate image degradation.

Related Experiment Videos