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Updated: May 19, 2026
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated
Jolieke G van Oosterwijk1, Danielle de Jong, Maayke A J H van Ruler
1Department of Pathology, Leiden University Medical Center, Albinusdreef 2, 2333, ZA, Leiden, The Netherlands.
Background:
Chondrosarcoma is the second most common primary sarcoma of bone. High-grade conventional chondrosarcoma and dedifferentiated chondrosarcoma have a poor outcome. In pre-clinical research aiming at the identification of novel treatment targets, the need for representative cell lines and model systems is high, but availability is scarce.
Methods:
We developed and characterized three cell lines, derived from conventional grade III chondrosarcoma (L835), and dedifferentiated chondrosarcoma (L2975 and L3252) of bone. Proliferation and migration were studied and we used COBRA-FISH and array-CGH for karyotyping and genotyping. Immunohistochemistry for p16 and p53 was performed as well as TP53 and IDH mutation analysis. Cells were injected into nude mice to establish their tumorigenic potential.
Results:
We show that the three cell lines have distinct migrative properties, L2975 had the highest migration rate and showed tumorigenic potential in mice. All cell lines showed chromosomal rearrangements with complex karyotypes and genotypic aberrations were conserved throughout late passaging of the cell lines. All cell lines showed loss of CDKN2A, while TP53 was wild type for exons 5-8. L835 has an IDH1 R132C mutation, L2975 an IDH2 R172W mutation and L3252 is IDH wild type.
Conclusions:
Based on the stable culturing properties of these cell lines and their genotypic profile resembling the original tumors, these cell lines should provide useful functional models to further characterize chondrosarcoma and to evaluate new treatment strategies.
Insights
We developed three new cell lines from high-grade chondrosarcoma (bone cancer) to aid in finding new treatments. These models accurately represent tumor genetics and will help researchers study this rare cancer.
Area of Science:
- Oncology
- Cancer Biology
- Genetics
Background:
- Chondrosarcoma is the second most common primary bone sarcoma.
- High-grade and dedifferentiated chondrosarcoma subtypes have poor prognoses.
- A scarcity of representative cell lines hinders pre-clinical research for novel treatment targets.
Purpose of the Study:
- To develop and characterize novel cell lines for chondrosarcoma research.
- To provide functional models for studying chondrosarcoma biology.
- To facilitate the evaluation of new therapeutic strategies for chondrosarcoma.
Main Methods:
- Development and characterization of three chondrosarcoma cell lines (L835, L2975, L3252).
- Assessment of proliferation, migration, karyotyping (COBRA-FISH, array-CGH), and genotyping.
- Immunohistochemistry for p16/p53, TP53/IDH mutation analysis, and in vivo tumorigenicity studies in mice.
Main Results:
- The cell lines exhibited distinct migratory properties, with L2975 showing the highest rate and tumorigenic potential.
- All cell lines displayed chromosomal aberrations and conserved genotypic profiles across passages.
- Loss of CDKN2A was observed in all lines; TP53 was wild type for critical exons, with specific IDH mutations identified (L835: IDH1 R132C, L2975: IDH2 R172W).
Conclusions:
- The developed chondrosarcoma cell lines possess stable culture properties and relevant genotypic profiles.
- These cell lines serve as valuable functional models for further chondrosarcoma characterization.
- The models are expected to aid in the evaluation of novel treatment strategies for chondrosarcoma.
