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Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...
Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

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Related Experiment Video

Updated: May 19, 2026

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
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Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Published on: August 21, 2021

Accelerated wound healing with topical application of complement C5.

Hani Sinno1, Meenakshi Malhotra, Justyn Lutfy

  • 1Montreal, Quebec, Canada From the Divisions of Plastic Surgery, Department of Surgery, and the Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, the Department of Pathology, and the Orthopedic Research Laboratory, McGill University.

Plastic and Reconstructive Surgery
|August 30, 2012
PubMed
Summary
This summary is machine-generated.

Topical application of complement C5 (a cytokine) significantly enhances wound healing, increasing skin wound strength within days. This study shows C5 accelerates healing by promoting collagen deposition and cell migration.

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Area of Science:

  • Wound Healing Research
  • Biomedical Science
  • Dermatology

Background:

  • Delayed wound healing poses significant challenges for patients and healthcare systems.
  • Investigating novel therapeutic targets for wound repair is crucial.
  • Complement C5, a cytokine, is explored for its potential role in wound healing.

Purpose of the Study:

  • To evaluate the efficacy of topical complement C5 in accelerating skin wound healing.
  • To quantify the impact of C5 on wound strength and tissue composition.

Main Methods:

  • Utilized a rat paired skin incision model for controlled study.
  • Applied topical C5 in a collagen vehicle to one incision, collagen alone to the contralateral incision.
  • Assessed wound breaking strength and analyzed collagen and fibronectin content at 3, 7, and 28 days post-wounding.

Main Results:

  • Topical C5 significantly increased maximum wound breaking strength by 65% at day 3 (p < 0.01) and 14% at day 7 (p < 0.05).
  • C5-treated wounds showed 83% greater strength at day 3 and 64% at day 7 compared to sham controls.
  • Western blot confirmed increased collagen and fibronectin deposition in C5-treated wounds, with no effect at 28 days.

Conclusions:

  • Topical C5 application demonstrably enhances wound healing, significantly increasing breaking strength from day 3 to day 7.
  • C5 accelerates healing by promoting vascular permeability, inflammatory cell recruitment, fibroblast migration, and collagen deposition.
  • The therapeutic effect of C5 on wound strength is most pronounced in the first week post-wounding.