Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spatial transcriptomic mapping of canine osteosarcoma reveals immune microenvironment features linked to survival.

Communications biology·2026

Same author

A murine model of sepsis induces age- and sex-specific chromatin remodeling in myeloid-derived suppressor cells.

Frontiers in immunology·2026

Same author

Divergent chromatin remodeling trajectories in CD66b <sup>+</sup> MDSCs distinguishes recovery from chronic critical illness after sepsis.

bioRxiv : the preprint server for biology·2026

Same author

Prolonged Loss of Oxidative Phosphorylation and Mitochondrial Mass Characterize CD66b<sup>+</sup> Leukocytes from Patients with Sepsis.

bioRxiv : the preprint server for biology·2025

Same author

CholeraSeq: a comprehensive genomic pipeline for cholera surveillance and near real-time outbreak investigation.

Bioinformatics (Oxford, England)·2025

Same author

Age- and Sex- Driven Transcriptional and Metabolic Diversity in Myeloid-Derived Suppressor Cells After Mouse Sepsis.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: May 19, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

DNA methyltransferase accessibility protocol for individual templates by deep sequencing.

Russell P Darst¹, Nancy H Nabilsi, Carolina E Pardo

¹Department of Biochemistry and Molecular Biology, University of Florida Shands Cancer Center Program in Cancer Genetics, Epigenetics, and Tumor Virology, Gainesville, Florida, USA.

Methods in Enzymology

|August 30, 2012

Summary

Single-molecule sequencing reveals dynamic chromatin states and epigenetic variants missed by bulk methods. This adaptation of the Methylation Accessibility Protocol for individual templates enhances chromatin structure analysis.

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Related Experiment Videos

Last Updated: May 19, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Area of Science:

Molecular Biology
Epigenetics
Genomics

Background:

Bulk chromatin analysis methods often miss dynamic states and rare epigenetic variants.
Single-molecule approaches offer higher resolution for studying chromatin structure.
Bisulfite genomic sequencing captures methylation status at multiple sites on individual DNA molecules.

Purpose of the Study:

To adapt the Methylation Accessibility Protocol for individual templates (MAPIT) for high-throughput sequencing.
To simplify the workflow and extend the utility of single-molecule chromatin analysis.
To enable the imaging of nucleosomes and protein-DNA complexes with greater efficiency.

Main Methods:

Adaptation of the Methylation Accessibility Protocol for individual templates (MAPIT).
Integration with modern high-throughput sequencing technologies.
Utilizing an exogenous DNA methyltransferase to probe protein-DNA interactions.

Main Results:

The adapted technique simplifies the workflow for single-molecule chromatin analysis.
The enhanced protocol extends the utility of MAPIT for studying chromatin structure.
High-throughput sequencing enables more efficient and comprehensive analysis of epigenetic variants.

Conclusions:

Single-molecule probing of chromatin structure provides critical insights beyond bulk methods.
The adapted MAPIT technique offers a powerful tool for investigating dynamic chromatin states.
This advancement facilitates the discovery of rare epigenetic variants with biological significance.