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Related Experiment Videos

Rabbit corticosteroid-binding globulin: primary structure and biosynthesis during pregnancy.

G E Seralini1, C L Smith, G L Hammond

  • 1Department of Obstetrics and Gynecology, University of Western Ontario, London, Canada.

Molecular Endocrinology (Baltimore, Md.)
|August 1, 1990
PubMed
Summary

Pregnancy significantly alters corticosteroid-binding globulin (CBG) levels in both mother and fetus. These changes are driven by increased CBG protein biosynthesis, not altered clearance rates.

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Area of Science:

  • Biochemistry
  • Genetics
  • Reproductive Biology

Background:

  • Corticosteroid-binding globulin (CBG) plays a crucial role in regulating steroid hormone bioavailability.
  • Understanding CBG regulation during pregnancy is vital for maternal and fetal health.
  • Previous studies have noted fluctuations in plasma CBG levels during gestation.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying changes in CBG levels during rabbit pregnancy.
  • To compare CBG gene expression in maternal and fetal tissues throughout gestation.
  • To determine if changes in CBG levels are due to altered biosynthesis or clearance.

Main Methods:

  • cDNA sequencing to determine the primary structure of rabbit CBG.
  • Northern blot analysis to quantify CBG mRNA levels in maternal and fetal tissues.

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  • Measurement of serum CBG levels in pregnant and non-pregnant rabbits.

    • Main Results:

    • Rabbit CBG primary structure deduced: 383 amino acids, 3 cysteine residues, 4 N-glycosylation sites.
    • CBG mRNA predominantly found in the liver, with smaller amounts in lung, spleen, ovary, and kidney.
    • Fetal hepatic CBG mRNA levels increased significantly after day 11, peaking and then declining by term.
    • Maternal hepatic CBG mRNA levels rose later in pregnancy, peaking near term, and returned to baseline postpartum.
    • Fetal and maternal CBG mRNA levels correlated with corresponding serum CBG concentrations.

    Conclusions:

    • Marked changes in maternal and fetal plasma CBG levels during pregnancy are primarily due to alterations in CBG protein biosynthesis.
    • These findings suggest a tightly regulated transcriptional control of CBG gene expression during gestation.
    • The study provides insights into the molecular basis of CBG regulation in the context of pregnancy.