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Updated: May 19, 2026

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Published on: September 17, 2008
Rapid casein quantification in milk powder with aggregation induced emission character of tetraphenylethene
Yingyi Liu1, Zhuo Wang, Guanxin Zhang
1CAS Key lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Zhongguancun, Beijing, PR China.
Abstract:
A casein assay has been developed with aggregation induced emission character of BSPOTPE, which is a derivative of tetraphenylethene (TPE). The method exhibits a wide dynamic range of detection for casein from 10 μg ml(-1) to 5000 μg ml(-1), and a good linear range from 20 μg ml(-1) to 1250 μg ml(-1). With sample-pretreatment procedures, we can detect the casein in skimmed and full milk powder, avoiding the interferences from other components and illegal additives in milk.
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