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[Screening of proteins regulated by CCDC19 gene in nasopharyngeal carcinoma using proteomics technology]
Weiyi Fang1, Qingping Jiang, Zhen Liu
1Cancer Research Institute, Southern Medical University, Guangzhou, China. fangweiyi1975@yahoo.com.cn
Objective:
To screen the proteins under regulation by the candidate tumor suppressor gene CCDC19 using proteomics technology in nasopharyngeal carcinoma (NPC).
Methods:
The cellular proteins were extracted from 3D8 NPC cells with CCDC19 overexpression and the control C6 NPC cells. Two-dimensional (2D) gel electrophoresis was employed to compare the protein expression profiles between these two cells, and the differential proteins were identified using peptide mass fingerprinting and database searching. Real-time PCR and Western blotting were used to validate the expression levels of the differential proteins.
Results:
Matrix-assisted laser desorption/time of flight showed that 3 differential proteins, namely FASN, CTSD and PGK1, were down-regulated by -3.28, -1.64, and -6.97 folds, respectively, which were confirmed by real-time PCR and Western blotting.
Conclusion:
FASN, CTSD and PGK1 are probably the target proteins regulated by CCDC19 in NPC.
