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[Determination of methyl isocyanate (MIC) in air of workplaces by HPLC]
Li-qun Song1, Yang Yu, Xiao-yun Wang
1Shandong Academy of Occupational Health and Occupational Medicine, Jinan, Shandong 250062, China.
Objective:
To establish the method of detecting the concentrations of methyl isocyanate (MIC) in air of workplaces with high performance liquid chromatographic (HPLC).
Methods:
Samples are collected by XAD-7 tubes coated with 1-(2-pyridyl) piperazine (1-2PP). Samples are desorbed with acetonitrile (ACN) and analyzed by high performance liquid chromatography (HPLC) using a fluorescence detector.
Results:
There was a linear relationship within the range of 0.01 ∼ 10 µg/ml, and the detection limit was 5.3×10(-4) µg/ml. The minimum detectable concentration was 0.01 µg/ml, and the lowest detected concentration was 3.3×10(-3) mg/m(3). The relative standard deviation was 2.2% ∼ 5.3%. The average desorption efficiency was 90% and the sampling efficiencies were 100%. The samples could be stored for 10 days in cold storage condition.
Conclusion:
The present method could meet with the requirements of Guide for establishing occupational health standards-Part4 Determination methods of air chemicals in workplace and be feasible for determination of MIC in workplace air.
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