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Updated: Jan 25, 2026
Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
Published on: February 3, 2023
[Effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1]
Peng Li1, Xiao-e Xiao, Zheng-tuan Guo
1Department of Pediatric Surgery, The Second Affliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China. sienafiat@hotmail.com
Objective:
To investigate the effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1.
Methods:
Hemangioma-derived endothelial cell line XTPS-1 was cultured with different concentration of bleomycin A5 (1000, 100, 10, 1, 0 mg/L), and then the survival rate was measured by methyl thiazolyl terazolium (MTT), the variation of cell morphology was observed using inverted phase contrast microscope and electron microscope, the variation of cell cycle and apoptosis rate were measured using flow cytometry.
Results:
After 24 hours culture the cell survival rate was (92.96 ± 3.66)% and (99.86 ± 0.12)% in lower saturation group (10 and 1 mg/L), but (34.08 ± 3.11)% and (43.28 ± 2.88)% in higher saturation group (1000 and 100 mg/L). The difference between them was more significant (P < 0.01). Lower saturation of bleomycin A5 (10 and 1 mg/L)could induce apoptosis but had almost no cytotoxic effect. Higher saturation of bleomycin A5 (1000 and 100 mg/L) not only induced apoptosis, but also had strong cytotoxic effect, which was concentration dependent.
Conclusions:
bleomycin A5 could induce apoptosis, inhibit cell proliferation and has direct cytotoxic effect.
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