Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides
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Published on: August 21, 2018
Vanessa Siegmund1, Tobias Santner, Ronald Micura
1Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
Chemical Communications (Cambridge, England)
|August 31, 2012
Abstract:
We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.
