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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
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A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
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Published on: December 16, 2015

Interphase Chromosome Flow-FISH.

Keyvan Keyvanfar1, Jason Weed, Prashanth Swamy

  • 1Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Blood
|August 31, 2012
PubMed
Summary
This summary is machine-generated.

A new Interphase Chromosome Flow-FISH (IC Flow-FISH) method efficiently detects monosomy 7 cells in myelodysplastic syndrome patients using flow cytometry. This technique offers a non-invasive approach, analyzing blood samples for chromosomal abnormalities.

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Area of Science:

  • Hematology
  • Cytogenetics
  • Molecular Biology

Background:

  • Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders.
  • Monosomy 7 is a common cytogenetic abnormality associated with MDS, often linked to poor prognosis.
  • Accurate detection of monosomy 7 is crucial for MDS diagnosis and treatment stratification.

Purpose of the Study:

  • To develop and validate a novel flow cytometry-based method for detecting monosomy 7 cells in MDS patients.
  • To establish a rapid and sensitive assay for identifying specific chromosomal abnormalities in blood samples.
  • To provide an alternative to traditional cytogenetic methods that may require bone marrow aspiration.

Main Methods:

  • Development of the Interphase Chromosome Flow-FISH (IC Flow-FISH) assay.
  • Utilizing flow cytometry and fluorescence in situ hybridization (FISH) with peptide nucleic acid (PNA) probes on intact interphase cells.
  • Analysis of peripheral blood leukocytes from MDS patients, involving cell fixation, permeabilization, and DNA hybridization.

Main Results:

  • The IC Flow-FISH method consistently detected hundreds to thousands of monosomy 7 cells in 10-20 mL of blood from MDS patients.
  • Results from IC Flow-FISH were compared with conventional cytogenetics, and monosomy 7 populations were verified using Fluorescence-Activated Cell Sorting (FACS).
  • Sensitivity testing by mixing patient and donor cells confirmed the assay's reliability.

Conclusions:

  • IC Flow-FISH is an effective 2-day method for detecting monosomy 7 in MDS patients.
  • This technique eliminates the need for bone marrow procurement and metaphase chromosome spreads.
  • The IC Flow-FISH methodology shows potential for broader applications in detecting other chromosomal abnormalities.