Related Experiment Video
Updated: May 19, 2026

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Published on: August 8, 2019
Adsorption behavior of plasmid DNA onto perfusion chromatographic matrix
Miladys Limonta1, Lourdes Zumalacárregui, Dayana Soler
1Center for Genetic Engineering and Biotechnology, Habana 10600, Cuba. miladys.limonta@cigb.edu.cu
Abstract:
Anion exchange chromatography is the most popular chromatographic method for plasmid separation. POROS RI 50 is a perfusion chromatographic support which is a reversed phase matrix and is an alternative to conventional ones due to its mass transfer properties. The adsorption and elution of the pIDKE2 plasmid onto reversed phase POROS R1 50 was studied. Langmuir isotherm model was adjusted in order to get the maximum adsorption capacity and the dissociation constant for POROS R1 50-plasmid DNA (pDNA) system. Breakthrough curves were obtained for volumetric flows between 0.69-3.33 mL/min, given dynamic capacity up to 2.3 times higher than those reported for ionic exchange matrix used during the purification process of plasmids with similar size to that of pIDKE2. The efficiency was less than 45% for the flow conditions and initial concentration studied, which means that the support will not be operated under saturation circumstances.
Related Concept Videos
Affinity Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Analyte Adsorption and Distribution

