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[Biological effect of micro ribonucleic acid-21 on human tongue squamous cell carcinoma lines]
Yin Wang1, Longjiang Li, Ke Xu
1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Objective:
To detect the biological influence to human tongue squamous cell carcinoma (TSCC) cells of micro ribonucleic acid-21 (miR-21).
Methods:
Referring to mature miR-21 sequence, the sense and antisense oligonucleotide (sense-miR-21 and AS-miR-21) modified by 2'O-Me were designed to transfect into TSCC cells (Tca8113 and high metastasis cells) by liposome transfection technology, in order to establish an in vitro TSCC cell model. The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polymerase chain reaction (real-time PCR). The changes of cell proliferation, cell cycle, cell early apoptosis, cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium (MTT), flow cytometry, Annexin V cell early apoptosis assay, scratch assay and Transwell assay, to check AS-miR-21's effect on the biological characteristics of human TSCC cell lines.
Results:
For the TSCC cells, the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation, promoted cell apoptosis, and inhibited the capability of cell's migration and invasion.
Conclusion:
The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells, and miR-21 can affect biological behavior of TSCC cells.
Insights
MicroRNA-21 (miR-21) significantly influences tongue squamous cell carcinoma (TSCC) progression. Inhibiting miR-21 with antisense oligonucleotides (AS-miR-21) reduced TSCC cell proliferation, migration, and invasion while promoting apoptosis.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Tongue squamous cell carcinoma (TSCC) is a prevalent oral cancer.
- MicroRNA-21 (miR-21) is implicated in various cancers, but its specific role in TSCC requires further elucidation.
- Understanding miR-21's biological influence is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the biological impact of microRNA-21 (miR-21) on human tongue squamous cell carcinoma (TSCC) cells.
- To establish an in vitro TSCC cell model for studying miR-21's effects.
- To assess the therapeutic potential of targeting miR-21 in TSCC.
Main Methods:
- Designed and transfected sense and antisense oligonucleotides (sense-miR-21 and AS-miR-21) into TSCC cell lines (Tca8113 and high metastasis cells) using liposome technology.
- Quantified miR-21 expression changes via real-time fluorescence quantitative polymerase chain reaction (real-time PCR).
- Evaluated cell proliferation (MTT assay), cell cycle, apoptosis (flow cytometry, Annexin V assay), migration (scratch assay), and invasion (Transwell assay).
Main Results:
- Antisense oligonucleotide targeting miR-21 (AS-miR-21) effectively inhibited TSCC cell proliferation.
- AS-miR-21 treatment promoted early apoptosis in TSCC cells.
- The capability of cell migration and invasion was significantly inhibited by AS-miR-21 in TSCC cells.
Conclusions:
- Transfection of AS-miR-21 led to decreased miR-21 expression in TSCC cells.
- miR-21 plays a significant role in regulating the biological behavior of TSCC cells.
- Targeting miR-21 presents a potential therapeutic strategy for tongue squamous cell carcinoma.
