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Updated: May 19, 2026
Creation of Abdominal Adhesions in Mice
Published on: August 27, 2016
Macroscopic and pathological assessment of methylene blue and normal saline on postoperative adhesion formation in a
Farzad Panahi1, Seyed Homayoon Sadraie, Hadi Khoshmohabat
1Trauma Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.
Background:
Adhesion formation after abdominal surgery is a major cause of postoperative bowel obstruction, infertility, and chronic abdominal pain. In this study, we evaluated the effect of normal saline and methylene blue (MB) on postoperative adhesion formation in a rat cecum model.
Methods:
A total of 30 Wistar female rats in 2 treatment and 1 control groups underwent midline laparotomy and standardized abrasion of the visceral peritoneum. Normal saline and methylene blue were administrated intraperitoneally at the end of the surgical procedure in 2 treatment groups. Fourteen days after surgery, a re-laparotomy was performed for macroscopic and pathological assessment.
Results:
The adhesion grade and extent of the normal saline group was lower than control and MB groups in macroscopic assessment (P<0.05 for both). A comparison of adhesion stages in pathological assessment showed increment in abdominal adhesion by usage methylene blue 1% and demonstrated significant difference between MB and 2 other groups (P<0.05).
Conclusions:
Administrated normal saline individually reduce the adhesion grade near cecum. Conversely, usage of methylene blue 1% may unpredictably increase risk of adhesion formation.
