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β-Pinene inhibited germination and early growth involves membrane peroxidation
Nadia Chowhan1, Harminder Pal Singh, Daizy R Batish
1Department of Botany, Panjab University, Chandigarh, 160014, India.
Beta-pinene, a natural compound, effectively inhibits weed growth by damaging cell membranes. This phytotoxicity impacts germination and root/shoot development in grassy and broad-leaved weeds.
Area of Science:
- Plant Science
- Agrochemistry
- Environmental Science
Background:
- Beta-pinene is a naturally occurring monoterpene found in essential oils.
- It is abundant in the environment and widely present in plants.
Purpose of the Study:
- To investigate the phytotoxicity of beta-pinene on weed germination and growth.
- To explore the mechanism of action, focusing on membrane integrity.
Main Methods:
- Tested phytotoxicity of beta-pinene on Phalaris minor, Echinochloa crus-galli, and Cassia occidentalis.
- Assessed germination, root, and shoot length inhibition.
- Evaluated membrane integrity via lipid peroxidation, electrolyte leakage, and enzyme activity (LOX, POX).
Main Results:
- Beta-pinene inhibited germination, root, and shoot growth in a dose-dependent manner.
- Grassy weeds and root growth were more sensitive to beta-pinene.
- Increased electrolyte leakage, malondialdehyde, hydrogen peroxide, and lipoxygenase activity indicated membrane damage.
Conclusions:
- Beta-pinene's phytotoxicity stems from disrupted membrane integrity, leading to peroxidation and loss of function.
- Enhanced peroxidases activity could not fully counteract beta-pinene-induced membrane damage.
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