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Related Experiment Video

Updated: May 19, 2026

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
15:40

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Published on: January 24, 2011

Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex.

Gerard M J Beaudoin1, Seung-Hye Lee, Dipika Singh

  • 1Department of Physiology, University of California-San Francisco (UCSF), San Francisco, California, USA.

Nature Protocols
|September 1, 2012
PubMed
Summary

We developed a protocol for culturing mouse hippocampal and cortical neurons. This method supports neuronal survival, development, and synaptic function for up to four weeks, aiding neuroscience research.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Culturing postnatal mouse neurons is crucial for studying genetically engineered models.
  • Existing methods may have limitations in supporting long-term neuronal health and functionality.

Purpose of the Study:

  • To present an optimized protocol for isolating and culturing early postnatal mouse hippocampal and cortical pyramidal neurons.
  • To enable robust neuronal cultures for various downstream applications in neuroscience research.

Main Methods:

  • Isolation and low-density dissociation of pyramidal neurons from postnatal day 0-1 mouse hippocampus and cortex.
  • Culture on poly-L-lysine-coated glass substrates without feeder layers.
  • Preparation of glass substrates initiated 5 days prior to neuronal plating.

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Isolation and Culture of Hippocampal Neurons from Prenatal Mice
10:27

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Published on: July 26, 2012

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
09:51

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons

Published on: January 29, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
15:40

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Published on: January 24, 2011

Isolation and Culture of Hippocampal Neurons from Prenatal Mice
10:27

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Published on: July 26, 2012

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
09:51

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons

Published on: January 29, 2015

Main Results:

  • Cultured neurons exhibit excellent survival, extensive axonal and dendritic arborization, and expression of neuronal/synaptic markers.
  • Functional synaptic connections are formed in vitro.
  • Neurons are amenable to low- and high-efficiency transfection and time-lapse imaging.

Conclusions:

  • This optimized cell culture technique provides a reliable method for maintaining mouse hippocampal and cortical neurons for up to 4 weeks.
  • The protocol supports diverse applications including immunocytochemistry, biochemical assays, shRNA knockdown, and live imaging.