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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

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Related Experiment Video

Updated: May 19, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

The Akt-associated microRNAs.

Min Xu1, Yin-Yuan Mo

  • 1Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu, People's Republic of China.

Cellular and Molecular Life Sciences : CMLS
|September 1, 2012
PubMed
Summary
This summary is machine-generated.

MicroRNAs regulate genes and are implicated in cancer. The Akt-microRNA network is crucial for cancer therapy, influencing Akt activity and microRNA regulation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are master gene regulators involved in cellular pathways.
  • Dysregulated miRNAs are linked to various human diseases, including cancer, where they can act as oncogenes or tumor suppressors.
  • Emerging evidence highlights the interconnection between miRNA-mediated gene regulation and the Akt pathway, forming a critical regulatory network.

Purpose of the Study:

  • To review recent advances in understanding the Akt-microRNA regulatory network.
  • To explore how microRNAs influence Akt activity and how Akt signaling regulates microRNAs.
  • To discuss the clinical implications of Akt-associated microRNAs in cancer therapy.

Main Methods:

  • Literature review of recent scientific publications.
  • Analysis of studies investigating microRNA and Akt pathway interactions.
  • Synthesis of information on the functional roles and clinical relevance of the Akt-microRNA network.

Main Results:

  • MicroRNAs and the Akt pathway form a complex regulatory network.
  • This network plays a significant role in cellular functions and disease processes, particularly in cancer.
  • Understanding this network is key to developing targeted cancer therapies.

Conclusions:

  • The Akt-microRNA regulatory network is a critical area of study for cancer biology.
  • Targeting this network holds promise for novel therapeutic strategies in oncology.
  • Further research is needed to fully elucidate the clinical implications of Akt-associated microRNAs.