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Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

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Related Experiment Video

Updated: May 19, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function.

Laura G Schuettpelz¹, Priya K Gopalan, Felipe O Giuste

¹Department of Pediatrics, Washington University, St Louis, MO, USA.

|September 1, 2012

Summary

Kruppel-like factor 7 (KLF7) is not essential for normal blood cell development. However, elevated KLF7 levels inhibit myeloid cell growth and support early T-cell survival, impacting leukemia outcomes.

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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

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Published on: February 21, 2018

Area of Science:

Hematology
Molecular Biology
Cancer Research

Background:

Kruppel-like factor 7 (KLF7) overexpression correlates with poor prognosis in pediatric acute lymphoblastic leukemia.
The role of KLF7 in normal hematopoiesis remains largely uncharacterized.

Purpose of the Study:

To investigate the functional role of KLF7 in murine hematopoiesis.
To determine the effects of KLF7 loss and enforced expression on hematopoietic stem and progenitor cells.

Main Methods:

Utilized knockout (Klf7(-/-)) and overexpression models in mice.
Assessed hematopoietic stem cell self-renewal via serial transplantation.
Analyzed progenitor cell proliferation and repopulating activity.
Performed RNA expression profiling of KLF7-overexpressing progenitors.

Main Results:

Loss of KLF7 did not affect long-term multilineage engraftment or self-renewal capacity.
Enforced KLF7 expression suppressed myeloid progenitor growth and repopulating activity.
KLF7 overexpression inhibited hematopoietic stem cells and common lymphoid progenitors but enhanced early thymocyte survival.
Cdkn1a (p21(Cip1/Waf1)) was not induced by KLF7, and its absence did not rescue the repopulating defect.

Conclusions:

KLF7 is dispensable for normal hematopoietic stem and progenitor cell function.
Elevated KLF7 expression inhibits myeloid proliferation and promotes thymocyte survival, potentially contributing to leukemia pathogenesis.
KLF7 may represent a therapeutic target in specific lymphoid leukemias.