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Updated: May 19, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
[Identification of a new heterotrophic nitrobacterium strain Colloides sp. JZ1-1 and its nitrifying capability]
Ji-Bo Xiao1, Hui-Xia Jiang, Shu-Yi Chu
1School of Environment and Resource, Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang, China. jbx958@yahoo.com.cn
Abstract:
A heterotrophic nitrobacterium strain JZ1-1 with higher nitrifying capability was isolated and mutagenized from an acclimated activated sludge. The JZ1-1 was identified as Colloides sp., according to its morphological and physiological features. The factors affecting the nitrifying capability of JZ1-1 were investigated, including medium carbon source, C/N ratio, pH value, dissolved oxygen, temperature, and ammonium nitrogen concentration. The optimal carbon source was sodium citrate, and the nitrification was favored when the C/N ratio was from 10 to 14, temperature was 30 degrees C, and pH value was 6-9 when cultured at a rotating speed of more than 150 r x min(-1). JZ1-1 could degrade ammonium nitrogen effectively when the initial concentration of ammonium nitrogen was from 100 mg x L(-1) to 500 mg x L(-1). JZ1-1 was stable after 5 generations of subculture.
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