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Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
Red cell and platelet-derived microparticles are increased in G6PD-deficient subjects
Duangdao Nantakomol1, Attakorn Palasuwan, Mallika Chaowanathikhom
1Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand. nantadao@gmail.com
European Journal of Haematology
|September 4, 2012
Summary
Individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency produce significantly more microparticles (MPs), which are cellular fragments linked to oxidative stress. This increase in MPs correlates with the severity of G6PD deficiency.
Area of Science:
- Cell Biology
- Hematology
- Biochemistry
Background:
- Cells shed microparticles (MPs) under oxidative stress and apoptosis.
- Glucose-6-phosphate dehydrogenase (G6PD) deficiency sensitizes cells to oxidative damage, potentially increasing MP formation.
Purpose of the Study:
- To investigate if G6PD deficiency alters membrane phospholipid asymmetry and elevates MP production.
- To quantify and determine the cellular origins of MPs in individuals with G6PD deficiency.
Main Methods:
- Flow cytometry was employed to measure circulating MP concentrations and cellular origins.
- Atomic Force Microscopy was utilized to analyze MP morphology and dimensions.
Main Results:
- G6PD-deficient individuals exhibited significantly higher circulating MP concentrations compared to G6PD-normal individuals.
- MP concentrations increased with the severity of G6PD deficiency and showed a strong correlation with G6PD enzymatic activity (r² = 0.731).
- MPs in G6PD deficiency were predominantly derived from red blood cells (45%) and platelets (30%).
Conclusions:
- MP concentration is significantly correlated with G6PD enzymatic activity.
- G6PD deficiency is associated with increased MP production, suggesting membrane alterations.
- The clinical significance of elevated MPs in G6PD deficiency requires further investigation.
