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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
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Study of antibody/antigen binding kinetics by total internal reflection ellipsometry.

Ieva Baleviciute1, Zigmas Balevicius, Asta Makaraviciute

  • 1Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania. zbalevicius@ar.fi.lt

Biosensors & Bioelectronics
|September 4, 2012
PubMed
Summary

Total internal reflection ellipsometry (TIRE) reveals distinct kinetics for biosensing layer formation. Antibody structure (intact vs. fragmented) significantly impacts antigen interaction residence time, crucial for sensor development.

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