Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Transduction01:16

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptidyl arginine deiminase 2 (PAD2) inhibition is associated with acute and long-term benefits in a murine model of traumatic brain injury.

The journal of trauma and acute care surgery·2026
Same author

Low-input CSF cfDNA shallow whole-genome sequencing for CNV-based diagnosis and monitoring of leptomeningeal metastasis in lung cancer.

Acta neuropathologica communications·2026
Same author

An integrated method for railway fastener defect detection and geometric parameter measurement using 3D line laser sensor.

PloS one·2026
Same author

PRV-delgE/gI/TK immunization preserves blood-brain barrier integrity and limits CNS injury following intracerebral PRV challenge.

Microbiology spectrum·2026
Same author

Author Correction: A proteogenomic atlas of 1032 brain metastases identifies molecular subtypes, immune landscapes, and therapeutic vulnerabilities.

Nature communications·2026
Same author

Genomic characterization and recombination analysis of hepatitis E virus in humans and swine across Asia: implications for food safety.

Frontiers in microbiology·2026

Related Experiment Video

Updated: May 19, 2026

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

Creating a "pro-survival" phenotype through epigenetic modulation.

Yongqing Li1, Baoling Liu, Xuesong Gu

  • 1Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Surgery
|September 4, 2012
PubMed
Summary
This summary is machine-generated.

Histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) treatment reduced inflammation and improved survival in rodent models of lipopolysaccharide (LPS)-induced endotoxic shock by modulating gene expression.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Related Experiment Videos

Last Updated: May 19, 2026

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Area of Science:

  • Immunology
  • Molecular Biology
  • Pharmacology

Background:

  • Lipopolysaccharide (LPS)-induced endotoxic shock is a life-threatening condition.
  • Histone deacetylase inhibitors, such as suberoylanilide hydroxamic acid (SAHA), have shown promise in improving survival rates.
  • The underlying molecular mechanisms of SAHA's protective effects remain largely undefined.

Purpose of the Study:

  • To investigate the impact of SAHA treatment on gene expression profiles during the early stages of LPS-induced endotoxic shock.
  • To identify specific genes and pathways modulated by SAHA in this model.
  • To explore potential therapeutic targets for endotoxic shock.

Main Methods:

  • Rodent model of LPS-induced endotoxic shock treated with or without SAHA.
  • Gene expression analysis using microarray on lung tissue harvested 3 hours post-LPS.
  • Validation of key gene expression changes using reverse transcriptase polymerase chain reaction and Western blotting.
  • Histological analysis of lung tissue to assess acute lung injury and neutrophil infiltration.
  • Gene network analysis to determine biological relevance and interactions.

Main Results:

  • LPS insult significantly altered the expression of numerous genes, including those not previously implicated in sepsis.
  • SAHA treatment attenuated the expression of key inflammatory genes and reduced neutrophil infiltration and acute lung injury in the lungs.
  • SAHA specifically inhibited genes involved in both the cellular and humoral arms of the innate immune system.
  • Gene network analysis revealed potential targets for novel therapeutic strategies.

Conclusions:

  • SAHA administration rapidly modulates gene transcription in LPS-induced shock, attenuating inflammatory mediators.
  • SAHA's effects on both cellular and humoral innate immunity may underlie its survival-promoting mechanism.
  • This study provides insights into the molecular mechanisms of SAHA in endotoxic shock, highlighting its potential as a therapeutic agent.