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Published on: September 11, 2020
Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection
Chien Chun Wang1, Lorena Sombra, Liliana Fernández
1INQUISAL-CONICET, San Luis, Argentina.
Talanta
|September 4, 2012
Summary
This study introduces a new method using Amberlite XAD-1180 resin and hexadecyltrimethylammonium bromide (HTAB) for enhanced pre-concentration and fluorescent detection of sildenafil. The technique significantly improves sensitivity for analyzing sildenafil in various samples.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Sildenafil analysis requires sensitive and efficient pre-concentration techniques.
- Surfactant-mediated methods can enhance the detection of pharmaceutical compounds.
Purpose of the Study:
- To develop an on-line pre-concentration method for sildenafil using Amberlite XAD-1180 resin and a cationic surfactant.
- To improve the sensitivity and applicability of sildenafil detection through spectrofluorimetry.
Main Methods:
- On-line pre-concentration of sildenafil on Amberlite XAD-1180 resin activated with hexadecyltrimethylammonium bromide (HTAB).
- Elution of retained sildenafil with ethanol.
- Quantitative analysis using spectrofluorimetry.
Main Results:
- Achieved a detection limit of 0.2 ng mL(-1) for sildenafil.
- Demonstrated significant fluorescence enhancement due to drug-surfactant association.
- Successfully applied the method to analyze sildenafil in bulk drug, urine, tablets, and herbal medicine.
Conclusions:
- The developed surfactant-mediated pre-concentration method offers high sensitivity and efficiency for sildenafil determination.
- This approach is robust and applicable to diverse real-world samples.
- The method provides a valuable tool for pharmaceutical and clinical analysis of sildenafil.
