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Updated: May 19, 2026
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
A fluorescence switch sensor for 6-mercaptopurine detection based on gold nanoparticles stabilized by
Zhanguang Chen1, Guomin Zhang, Xi Chen
1Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn
Abstract:
It is of great significance to develop a simple and powerful assay of 6-mercaptopurine (6-MP) because of its serious side effect and variable activity with the plasma concentration. In this contribution, a fluorescence switch sensor for trace amount detection of 6-MP was successfully developed based on the fluorescent gold nanoparticles stabilized by macromolecules. With the turn-off and on of the fluorescence signal at 640 nm of the analytical system, the selectivity of the present assay was largely improved. Trace amount of 6-MP could be detected in the linear range 1.0×10(-7) M-1.2×10(-4) M with a detection limit 1.98×10(-8) M. Under a UV lamp, the color change with the variation of the 6-MP concentration could be seen clearly by naked eyes. The sensitivity and selectivity are several-fold greater than other methods. And also it proved to be able to detect trace amount of 6-MP in real samples. The present assay largely improved the application of spectral methods in quantitative analysis of 6-MP.
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