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Published on: October 24, 2016
Enhanced bioethanol production from pretreated corn stover via multi-positive effect of casein micelles
Anahita Dehkhoda Eckard1, Kasiviswanathan Muthukumarappan, William Gibbons
1Department of Agricultural and Biosystems Engineering, 1400 North Campus Drive, South Dakota State University, Brookings, SD 57007, USA. Anahita.Eckard@sdstate.edu
Abstract:
Casein polypeptides containing substructures of αs1-casein, β-casein, k-casein, αs2-casein were used as a lignin-blocker at above critical micelles concentration to improve the bioethanol production of dilute acid, lime, alkali, extrusion and AFEX pretreated corn stover (CS). Application of 0.5 g/g glucan of casein was found to effectively increase the glucose yield of CS pretreated with dilute acid, lime, alkali, extrusion and AFEX by 31.9%, 17.0%, 22.7%, 29.5%, and 17.4%, respectively with no positive impact on Avicel. Consequently 96 h simultaneous saccharification and fermentation (SSF) of these hydrolysates reduced the fermentation period by up to 48 h and increased the theoretical yield of ethanol by 8.48-33.7% compared to control. Application of casein during saccharification reduced the enzyme utilization by 33.0%. Recycling of hydrolysate from casein-treated CS for a 2nd round hydrolysis resulted in average glucose yield of 36.4% compared to 29.0% control.
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