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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: May 19, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Singles engage the RNA interference pathway.

Beverly L Davidson, Alex Mas Monteys

    Cell
    |September 4, 2012
    PubMed
    Summary
    This summary is machine-generated.

    Chemically modified single-stranded RNAs can effectively induce gene silencing, matching the performance of double-stranded RNAs. This breakthrough offers a new avenue for RNA interference applications.

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    Last Updated: May 19, 2026

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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    Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
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    Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

    Published on: September 5, 2016

    Area of Science:

    • Molecular Biology
    • RNA Interference
    • Gene Regulation

    Background:

    • Single-stranded RNAs (ssRNAs) are known to interact with the RNA interference (RNAi) pathway.
    • The RNAi pathway is a conserved biological process that regulates gene expression by targeting messenger RNAs (mRNAs).
    • Typically, double-stranded RNAs (dsRNAs) are more potent inducers of RNAi-mediated gene silencing compared to ssRNAs.

    Discussion:

    • This study investigates the potential of chemically modified ssRNAs to act as effective gene silencing agents.
    • Researchers explored the structural and chemical modifications required to enhance the efficacy of ssRNAs in the RNA interference pathway.
    • The findings demonstrate that modified ssRNAs can achieve gene silencing with an efficiency comparable to dsRNAs.

    Key Insights:

    • Extensive chemical modifications can transform ssRNAs into robust inducers of gene silencing.
    • Modified ssRNAs exhibit comparable efficacy to dsRNAs in reducing target mRNA expression.
    • This research expands the toolkit for RNA interference-based gene modulation.

    Outlook:

    • Further research may explore the therapeutic applications of these modified ssRNAs.
    • Investigating the stability and delivery of modified ssRNAs for in vivo applications.
    • Exploring the precise mechanisms by which modified ssRNAs engage the RNA interference machinery.