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Updated: May 19, 2026
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human
Christian J Gaffney1, Tsutomu Oka, Virginia Mazack
1Laboratory of Signal Transduction and Proteomic Profiling, Weis Center for Research, Geisinger Clinic, 100 North Academy Avenue, Danville, PA 17821-2608, USA.
Gene
|September 4, 2012
Summary
Researchers identified 4 new YAP1 mRNA splicing isoforms, totaling 8. These variants, with differing expression in human tissues and evolutionary origins, impact YAP1 protein structure and signaling pathways.
Area of Science:
- Molecular Biology
- Genetics
- Evolutionary Biology
Background:
- The YAP1 gene acts as an oncogene and stem cell factor, but can function as a tumor suppressor in certain cancers.
- YAP1 gene products are extensively studied and utilized in cellular and animal models.
Purpose of the Study:
- To report novel mRNA splicing isoforms of the YAP1 gene.
- To analyze the expression patterns and evolutionary origins of YAP1 isoforms.
- To investigate the structural and functional consequences of YAP1 alternative splicing.
Main Methods:
- Detection of YAP1 mRNA isoforms in human tissues.
- Real-Time PCR for quantifying YAP1-2δ expression.
- Comparative genomic analysis of YAP1 in metazoans.
Main Results:
- Identified 4 new YAP1 mRNA splicing isoforms, bringing the total to 8.
- Detected all 8 isoforms across human tissues, with low expression in leukocytes.
- Found that YAP1 isoforms utilizing exon 6 evolved with amniotes.
- Observed that several isoforms disrupt the leucine zipper region, impacting YAP1 signaling.
Conclusions:
- A total of 8 YAP1 mRNA splicing isoforms have been identified.
- Differential expression and evolutionary patterns of YAP1 isoforms suggest functional diversification.
- Alternative splicing of YAP1 impacts protein structure, particularly the leucine zipper domain, with implications for signaling pathways.
- A standardized nomenclature for YAP1 variants is proposed to facilitate research.
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