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Updated: May 19, 2026

Murine Model of CD40-activation of B cells
Published on: March 5, 2010
Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin
Lisa A Kohn1, Qian-Lin Hao, Rajkumar Sasidharan
1Department of Pathology & Laboratory Medicine, University of California, Los Angeles, California, USA.
Abstract:
Expression of the cell-surface antigen CD10 has long been used to define the lymphoid commitment of human cells. Here we report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before onset of the expression of CD10 and commitment to the B cell lineage. We identified this subset by high expression of the homing molecule L-selectin (CD62L). CD10(-)CD62L(hi) progenitors had full lymphoid and monocytic potential but lacked erythroid potential. Gene-expression profiling placed the CD10(-)CD62L(hi) population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin(-)) CD34(+)CD10(+) progenitors. CD62L was expressed on immature thymocytes, and its ligands were expressed at the cortico-medullary junction of the thymus, which suggested a possible role for this molecule in homing to the thymus. Our studies identify the earliest stage of lymphoid priming in human bone marrow.
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