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High prevalence of the B2+C2 subgenotype mixture in patients with chronic hepatitis B in Eastern China

Jun Zhong1, Yue-Qiu Gao, Xue-Hua Sun

  • 1Shanghai University of Traditional Chinese Medicine Affiliated Shanghai Shuguang Hospital, Department of Infectious Diseases, Key Laboratory of Liver and Kidney Diseases, Shanghai University of Traditional Chinese Medicine, Ministry of Education E-institute of Shanghai Municipal Education Commission, Shanghai, China.

Acta Pharmacologica Sinica
|September 4, 2012
PubMed

Insights

Hepatitis B virus (HBV) genotype mixtures, particularly B2+C2, are common in Eastern China. This HBV B2+C2 subgenotype is linked to higher viral replication and a more severe disease progression in chronic hepatitis B patients.

Area of Science:

  • Virology
  • Hepatology
  • Infectious Diseases

Background:

  • Chronic hepatitis B (CHB) affects millions globally.
  • Understanding HBV genotype distribution is crucial for disease management.
  • Genotype mixtures may influence disease progression and treatment outcomes.

Purpose of the Study:

  • To determine the prevalence of hepatitis B virus (HBV) genotype mixtures in Eastern China.
  • To analyze the association between HBV genotypes, viral load, and disease severity.
  • To investigate the characteristics of specific HBV subgenotypes, such as B2+C2.

Main Methods:

  • Enrollment of 4908 CHB patients from Eastern China.
  • Multiplex PCR for HBV genotype and subgenotype determination.
  • Real-time PCR and ELISA for viral load and HBeAg quantification.
  • PCR and direct sequencing for precore/basic core promoter (PC/BCP) mutations.

Main Results:

  • HBV genotypes B, C, D, B+C, and B+D were detected in 19.21%, 64.75%, 1.49%, 13.63%, and 0.92% of patients, respectively.
  • The B+C genotype mixture was more frequent in moderate CHB patients.
  • HBV B2+C2 subgenotype showed higher viral loads and was associated with more severe CHB, particularly in older age groups.
  • A double mutation (G1896A) in the PC was more common in B2+C2 compared to B2+C1.

Conclusions:

  • The HBV B2+C2 subgenotype is prevalent in CHB patients with high viral replication.
  • HBV B2+C2 is correlated with a more severe disease course.
  • Genotype mixtures, especially B2+C2, warrant further investigation for clinical implications.
Abstract

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