Coupling endonucleases with DNA end-processing enzymes to drive gene disruption
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Updated: May 19, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
Published on: June 8, 2018
Michael T Certo1, Kamila S Gwiazda, Ryan Kuhar
1Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA.
Abstract:
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.
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