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Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
Karyotype of asparagus by physical mapping of 45S and 5S rDNA by FISH
Chuan-Liang Deng1, Rui-Yun Qin, Ning-Na Wang
1College of Life Science, Henan Normal University, Xinxiang 453007, People's Republic of China.
Journal of Genetics
|September 4, 2012
Abstract
No abstract available in PubMed .
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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
